2.8.01 Sand Dollar Live



2.8.02 injecting sand dollars with postassium chloride making them release gametes



2.8.03 female sanddollar releasing eggs



2.8.04 Sand dollar eggs



2.8.05 newly fertilized egg with tiny sperm specs and the fertilization membrane



2.8.06 Elayne watching fertilization



2.8.07 using a special contrast lighting on the microscope showing the newly formed 2-cell stage



2.8.08 the 4 cell stage



2.8.09 8 cell stage- upper rt is in the process of dividing into the 16 cell stage



2.8.10 Travis showing his MacConkey's agar plate



2.8.11 MacConkey's agar is selective it grows only gram negative bacteria like E.coli



2.8.12 MacConkey's agar is also DIFFERENTIAL - showing a color difference btwn colonies that can break down the sugar lactose & those that cannot



2.8.13 the final set of MacConkey's designs



2.8.14 Replica Plating Results- top row Minimal Media where only competent cells can grow- bottom row Nutrient Agar grows most all bacteria